酶工程-(第三版) 本书特色
This book was translated from Chinese "EnzymeEngineering" (Third Edition) published in 2009 by Science Press,Beijing, China. The contents of the book include 10 chapters whichare; chapter 1: introduction; chapter 2. Enzyme production bymicrobial fermentation; chapter 3. Enzyme production by animal andplant cell culture; chapter 4. Extraction, isolation andpurification of enzymes; chapter 5. Enzyme molecular modifications;chapter 6. Immobilization of Enzymes, Cells and Protoplasts;chapter 7. Enzyme catalysis in non-aqueous phases; chapter 8.Enzyme directed evolutions; chapter 9. Enzyme reactors and chapter10. Applications of enzymes. 《酶工程(第3版)》由Guo Yong所著。
酶工程-(第三版) 内容简介
《酶工程(第3版)》简介:Enzyme Engineering (ThirdEdition) introduces the basic theories, basic technologies recentdevelopments and growth trends in the fields of enzyme productionand applications. This book in 10 chapters covers the introduction,enzyme production by microbial fermentation, enzyme production byanimal and plant cell cultures, extraction, separation andpurification of enzymes, enzyme molecular modification,immobilization of enzymes, cells and protoplasts, enzyme catalysisin non-aqueous phases, enzyme directed evolution, enzyme reactors,and applications of enzymes. A review and thinking issues are givenat the end of every chapter. This book can be used as a text bookfor the students specializing in biotechnology, bioengineering,biochemical engineering, enzyme engineering, fermentationengineering, bioscience etc. It can also be used as reference bythe teachers, scientists, engineers and technicians working in thefields related to enzyme engineering. This book has been translatedfrom the third Chinese edition of "Enzyme Engineering" published byScience Press, Beijing, China. 《酶工程(第3版)》由Guo Yong所著。
酶工程-(第三版) 目录
Preface
Chapter 1Introduction
1.1Conception of Enzyme and Its Development
1.2The Characteristics of Enzyme Action
1.3Factors that Affecting Enzyme Action
1.4Classification and Nomenclature of Enzymes
1.5Determination of Enzyme Activity
1.6Methods for Enzymes Production
1.7Development Survey of Enzyme Engineering
Review and Thinking
Chapter 2Enzyme Production by Microbial Fermentation
2.1Regulation of Enzyme Biosynthesis in MicrobialCells
2.2Characteristic of Microbes for EnzymeProduction
2.3Fermentation Process Conditions and ItsControl
2.4Enzyme Fermentation Kinetics
Preface
Chapter 1 Introduction
1.1 Conception of Enzyme and Its Development
1.2 The Characteristics of Enzyme Action
1.3 Factors that Affecting Enzyme Action
1.4 Classification and Nomenclature of Enzymes
1.5 Determination of Enzyme Activity
1.6 Methods for Enzymes Production
1.7 Development Survey of Enzyme Engineering
Review and Thinking
Chapter 2 Enzyme Production by Microbial Fermentation
2.1 Regulation of Enzyme Biosynthesis in MicrobialCells
2.2 Characteristic of Microbes for EnzymeProduction
2.3 Fermentation Process Conditions and ItsControl
2.4 Enzyme Fermentation Kinetics
2.5 Enzyme Production by Immobilized CellFermentation
2.6 Enzyme Production by Immobilized ProtoplastFermentation
Review and Thinking
Chapter 3 Enzyme Production by Animal and Plant CellCultures
3.1 The Regulation of Enzyme Biosynthesis in Animaland Plant Cells
3.2 Enzyme Production by Plant Cell Culture
3.3 Enzyme Production by Animal Cell Culture
Review and Thinking
Chapter 4 Extraction, Separation and Purification ofEnzymes
4.1 Cell Disruption
4.2 Enzyme Extraction
4.3 Precipitation Separation
4.4 Centrifugal Separation
4.5 Filtration and Membrane Separation
4.6 Chromatographic Separation
4.7 Eleetrophoresis Separation
4.8 Extraction Separation
4.9 Crystallization
4.10 Concentration and Drying
Review and Thinking
Chapter 5 Enzyme Molecular Modification
5.1 Metal Ion Substitution Modification
5.2 Macromolecular Binding Modification
5.3 Side Chain Group Modification
5.4 Peptide Chain Limited HydrolysisModification
5.5 Nucleotide Chain Cleavage Modification
5.6 Amino Acid Substitution Modification
5.7 Nucleotide Substitution Modification
5.8 Physical Modification
5.9 Application of Enzyme MolecularModification
Review and Thinking
Chapter 6 Immobilization of Enzymes, Cells andProtoplasts
6.1 Enzyme Immobilization
6.2 Cell Immobilization
6.3 Protoplast Immobilization
Review and Thinking
Chapter 7 Enzyme Catalysis in Non-aqueous Phases
7.1 The Main Scopes of Enzyme Catalysis inNon-aqueous Phase
7.2 Effects of Water and Organic Solvents on EnzymeCatalysis in Organic Media
7.3 The Catalytic Characters of Enzymes in OrganicMedia
7.4 Conditions of Enzyme Catalytic Reaction inOrganic Media and Its Control
7.5 Application of Enzyme Catalysis in Non-aqueousPhase
Review and Thinking
Chapter 8 Enzyme Directed Evolution
8.1 The characteristics of Enzyme DirectedEvolution
8.2 Random Mutation of Enzyme Gene
8.3 Directed Selection of Enzyme Gene Mutants
8.4 The Applications of Enzyme DirectedEvolution
Review and Thinking
Chapter 9 Enzyme Reactors
9.1 Types of Enzyme Reactors
9.2 Choice of Enzyme Reactors
9.3 Design of Enzyme Reactors
9.4 Enzyme Reactor Operation
Review and Thinking
Chapter 10 Applications of Enzymes
10.1 Enzymes Application in PharmaceuticalIndustry
10.2 Application of Enzymes in Food
10.3 Enzyme Application in Light and ChemicalIndustries
10.4 Enzyme Application in EnvironmentalProtection
10.5 Enzyme Application in Biotechnology
Review and Thinking
Main references
酶工程-(第三版) 节选
This book was translated from Chinese "EnzymeEngineering" (Third Edition) published in 2009 by Science Press,Beijing, China. The contents of the book include 10 chapters whichare; chapter 1: introduction; chapter 2. Enzyme production bymicrobial fermentation; chapter 3. Enzyme production by animal andplant cell culture; chapter 4. Extraction, isolation andpurification of enzymes; chapter 5. Enzyme molecular modifications;chapter 6. Immobilization of Enzymes, Cells and Protoplasts;chapter 7. Enzyme catalysis in non-aqueous phases; chapter 8.Enzyme directed evolutions; chapter 9. Enzyme reactors and chapter10. Applications of enzymes. 《酶工程(第3版)》由Guo Yong所著。
酶工程-(第三版) 作者简介
GUO Yong Professor,South China University of Technology
